IDH1/2 Mutations Detection Kit

Real-time
PCR

CE-IVD

-This kit uses Allele Specific Oligonucleotide (ASO) Real-time PCR based method.-Superior analytical sensitivity and specifity with Allele Spesfic PCR.

-IDH1 and 2 mutations have been acknowledged as good biomarkers and potential drug targets.

-Fast and Easy use with Multiplex Realtime PCR Technology.

-Can detect R132L, R132V, R132G, R132S, R132H, R132C, R132P, R100Q, R140G, R140L, R140W, R140Q, R172S, R172K, R172W, R172M, R172G mutations on IDH1/2 genes.

-Automatic analysis with geneMAP viewer (for CFX96)

INTENDED USE

The geneMAP™ IDH1/2 Mutations Detection Kit is intended for the detection of IDH1 and IDH2 mutations (listed in Table below) in human genomic DNA.

Gene	Exon	Amino Acid Change	Nucleotid Change	HGSV nt Change
IDH1	4	Wildtype	Wildtype	Wildtype
IDH1	4	R132L	c.395G>T	LRG_610t1:c.395G>T
IDH1	4	R132V	c.394_395 CG>GT	NM_005896.4:c.394_395 delinsGT
IDH1	4	R132G	c.394C>G	LRG_610t1:c.394C>G
IDH1	4	R132S	c.394C>A	LRG_610t1:c.394C>A
IDH1	4	R132H	c.395G>A	LRG_610t1:c.395G>A
IDH1	4	R132C	c.394C>T	LRG_610t1:c.394C>T
IDH1	4	R132P	c.395G>C	LRG_610t1:c.395G>C
IDH1	4	R100Q	c.299G>A	LRG_610t1:c.299G>A
IDH2	4	Wildtype	Wildtype	Wildtype
IDH2	4	R140G	c.418C>G	LRG_611t2:c.418C>G C
IDH2	4	R140L	c.419G>T	LRG_611t2:c.419G>T
IDH2	4	R140W	c.418C>T	LRG_611t2:c.418C>T
IDH2	4	R140Q	c.419G>A	NM_002168.4:c.419G> A
IDH2	4	R172S	c.516G>T	LRG_611t2:c.516G>T
IDH2	4	R172K	c.515G>A	LRG_611t2:c.515G>A
IDH2	4	R172W	c.514A>T	LRG_611t2:c.514A>T
IDH2	4	R172M	c.515G>T	LRG_611t2:c.515G>T
IDH2	4	R172G	c.514A>G	LRG_611t2:c.514A>G