DPYD Comprehensive Variant Detection Kit

Real-time
PCR

RUO

A multiplex real-time PCR that allows amplification of wild-type and mutant nucleic acids.
Allele Specific Realtime PCR (Aso-Realtime PCR)
Compatible with FAM and VIC/HEX dual color real-time PCR instruments.

INTENDED USE

The geneMAP™ DPYD Comprehensive Variant Detection Kit is an allele specific real-time PCR test designed to detect 7 tier one and 6 tier two variants, as defined by the Association for Molecular Pathology (AMP) joint consensus recommendation, of DPYD gene in genomic DNA of blood samples.

Tier	rs ID	Legacy Name	Nucleotide Change	Protein Change	Allele Function
Tier 1 Variants	rs3918290	*2A	c.1905+1G>A	N/A	No Function
	rs55886062	*13	c.1679T>G	p.Ile560Ser	No Function
	rs75017182	HapB3	c.1129-5923C>G	N/A	Decreased
	rs115232898	N/A	c.557A>G	p.Tyr186Cys	Decreased
	rs146356975	N/A	c.868A>G	p.Lys290Glu	Decreased
	rs112766203	N/A	c.2279C>T	p.Thr760Ile	Decreased
	rs67376798	N/A	c.2846A>T	p.Asp949Val	Decreased
Tier 2 Variants	rs72549309	*7	c.299_302del	p.Phe100fs	No Function
	rs1801266	*8	c.703C>T	p.Arg235Trp	No Function
	rs186169810	N/A	c.1314T>G	p.Phe438Leu	Decreased
	rs72549304	N/A	c.1475C>T	p.Ser492Leu	No Function
	rs59086055	N/A	c.1774C>T	p.Arg592Trp	No Function
	rs55674432	N/A	c.2639G>T	p.Gly880Val	No Function