The Realtime PCR (qPCR) DNA amplification technique is highly sensitive and specific. The kit is based on two major processes: nucleic acid extraction and PCR amplification of target nucleic acid using TaqMan Probes and primers with real-time PCR machine. geneMAPTM IDH1/2 Mutations Detection Kit is a multiplex real time PCR assay that permits amplification of target nucleic acids of mutated alleles and Endogenous Control (EC).