geneMAP™ MTB Detection Kit (MTB-RT100)
- Superior Analytical Sensitivity and specificity
- Fast and Easy to Use with Multiplex Realtime PCR Technology
- Contamination Preventing System (UDG)
- Compatible with FAM, VIC/HEX two Colors Real-Time PCR Instruments
- Includes DNA Extraction System
Tuberculosis (TB) remains a major public health problem worldwide. In 2015, 10.4 million new cases of TB were reported. Approximately 1.4 million people died of the disease, and there were an additional 0.4 million deaths from co-infected with HIV . Rapid diagnosis and treatment are pivotal for the effective control of TB in clinical practice. Acid-fast staining and Mtb culture are classical Mtb diagnosis techniques. The acid-fast stain lacks sensitivity, and the culture requires several weeks for incubation. The inherent limitations make it difficult for them to meet the requirement for early diagnostics
Rapid and accurate diagnosis of tuberculosis (TB) is key to manage the disease and to control and prevent its transmission. Many established diagnostic methods suffer from low sensitivity or delay of timely results and are inadequate for rapid detection of Mycobacterium tuberculosis (MTB) in pulmonary and extra-pulmonary clinical samples. This study examined whether a real-time polymerase chain reaction (RT-PCR) assay, with a turn-a-round time of 2 h, would prove effective for routine detection of MTB by clinical microbiology laboratories.