geneMAP™ BCR-ABL1 (p190, p210, p230) Screening Kit (BCRSCR-RT48)
- Superior Analytical Sensitivity and specificity with Quadruplex PCR
- Fast and Easy to Use with One Step RT-qPCR Technology
- Accurate Screening and genotyping with Multiplex PCR for the most common fusion transcripts of BCR-ABL1/ABL1 (p210, p190 & p230)
- Compatible with FAM, VIC/HEX, ROX and CY5 Four Colors Real-Time PCR Instruments
BCR-ABL1 translocation can be observed in myeloproliferative neoplasms, acute leukemia of ambiguous lineage and precursor lymphoid neoplasms class as chronic myeloid leukemia (CML), acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). Philadelphia chromosome (Ph chromo-some) is a shortened chromosome 22, resulting from translocation between long arms of chromosome 9 and 22 in hematopoietic stem cells, presented as t (9;22) (q34;q11). In addition, Ph chromo-some is found in over 90% of patients with CML. Molecular consequences are formation of BCR-ABL1 fusion gene on chromosome 22 and a reciprocal ABL-BCR on chromosome 9. The resulting BCR-ABL1 protein is located in the cytoplasm with a deregulated tyrosine kinase activity. Three main types of hybrid genes BCR-ABL1 are observed, producing three isoforms of tyrosine kinase protein with abnormal activity: p230 BCR-ABL p210 BCR-ABL, p190 BCR-ABL. p190BCR-ABL is an uncommon isoform in cases of CML and often observed in children with ALL. p210 BCR/ABL is present in most patients with CML in stable phase and some cases of ALL and AML.
The geneMAP™ BCR-ABL1 (p190, p210, p230) Screening assay for screening and genotyping of 3 most common (p190, p210 and p230) fusion transcripts in one PCR well with its one-step Quadruplex technology.