geneMAP™ JAK2 V617F Mutation Detection Kit (JK14-RT50)
- Superior Analytical Sensitivity and specificity with Allele Specific PCR
- Fast and Easy to Use with Multiplex Realtime PCR Technology
- Quantitative (Calculation of Allelic Burden %)
- Compatible with FAM, VIC/HEX two Colors Real-Time PCR Instruments
- Research Use Only (RUO)
Somatic mutation of the JAK2 gene (JAK2 p.V617F) can be detected in a variable proportion of patients with myeloproliferative neoplasms. The JAK2 p.V617F mutation is observed in as much as 96% of patients with polycythemia vera and approximately 55% to 65% of those with essential thrombocythemia and primary myelofibrosis. In addition, quantitative measurement of the JAK2 p.V617F allele burden (ratio of mutant allele to total allele) has been associated with certain clinical phenotypes, such as a higher incidence of pruritus and splenomegaly and an increased risk of thrombosis in patients with PV and ET, although the exact association between allele burden and long-term outcome remains controversial, particularly in patients with myelofibrosis.